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The antibody is from purified rabbit serum by affinity purification by means of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Overexpression of this gene has long been noticed in lots of tumors, which results in chromosome instability, and so might add to tumorigenesis. This protein was found to affiliate with, and become linked to, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle controlled histone gene expression and performs a significant purpose in selling cell-cycle development inside the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode unique isoforms, have already been explained. Two added splice variants had been reported but in-depth nucleotide sequence data isn't yet out there.

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Blocking peptides are peptides that bind exclusively on the focus on antibody and block antibody binding. These peptide normally is made up of the epitope acknowledged from the antibody.

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This guideline outlines even further possible optimization of cellular and immunoassay parameters to make certain the very best results are...

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This guidebook outlines more feasible optimization of mobile and immunoassay parameters to make sure the absolute best success are...

Antibodies certain to the blocking peptide not bind to your epitope over the focus on protein. By comparing the staining with the blocked antibody compared to the antibody on your own, you can see which staining is unique.

Concentrate on Facts Cyclin E belongs into the remarkably conserved cyclin relatives, whose members are characterized by a remarkable periodicity in protein abundance throughout the cell cycle. Cyclins perform as regulators of CDK kinases. Various cyclins show distinctive expression and degradation designs which contribute into the temporal coordination of each and every mitotic function. Cyclin E types a posh with and features like a regulatory subunit of CDK2, whose exercise is needed for cell cycle G1/S changeover. This protein accumulates with the G1-S section boundary and is also degraded as read more cells progress as a result of S stage.

The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both antibodies...

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